Recently, I had the chance to join an experiment at the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. Led by Dr. Elena Longo and Dr. Lorella Pascolo, our team examined human tissue at the cellular level, focusing on research related to reproductive medicine. The experiment took place at the ID16a beamline, renowned for its high-brilliance beam, which can be focused down to nanometer size. This incredible precision allows for the quantitative 3D characterization of the morphology and elemental composition of specimens in their native state. By combining coherent imaging techniques and X-ray fluorescence microscopy, ID16a provides unparalleled insights into the microstructures of various samples.
I was very happy to participate in this endeavor, as my research examines growth, development, and life history through an evolutionary lens. However, I had not previously explored how nano tomography can be used as an approach for virtual histology. We obtained excellent data and it was a very useful experience, in all domains such as project planning, sample preparation, sample positioning, acquisition modes and data reconstruction. This experience has been highly interesting and enriching, offering a fresh perspective to complement my research focus and enhancing my understanding of how nanotomography complements Microtomography.
Our work was greatly supported by the talented beamline scientists and postdoctoral researcher Marina Eckermann and Peter Cloetens who played a crucial role in the data acquisition. And most of all, big thanks to Elena and Lorella for asking me to join in their research project!